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. 2022 Sep 1;132(17):e159876. doi: 10.1172/JCI159876

Figure 5. No viral replication after culture of SARS-CoV2 RNA–positive human plasma in susceptible cell lines.

Figure 5

(A) Vero-TMPRSS2 cells in 96-well plates were infected with indicated doses of SARS-CoV-2. Five replicate wells were tested for each dosage. Two days after infection, weak CPE developed in all wells infected with as low as 7 PFU/well, and no CPE developed in all wells infected with 0.7 PFU/well (representative wells shown). (B) Vero-ACE2-TMPRSS2 cells were tested as in A. Two days after infection, clear CPE developed in all wells infected with as low as 0.7 PFU and 1 out of 5 wells infected with 0.07 PFU developed CPE. (C) Vero-ACE2-TMPRSS2 cells were incubated with 5 different SARS-CoV-2 RNA–positive human plasmas in 5 replicate wells each for 2 hours at 37°C before washing and incubation with fresh medium. No CPE developed in any wells at 3 days after infection.