Skip to main content
. 2022 Sep 1;132(17):e157250. doi: 10.1172/JCI157250

Figure 5. Combined FcγRIIB and MZ deficiency reverses the enhanced response to antigen challenge and the increase in autoantibody production.

Figure 5

(A–F) Female control, Notch2-cKO, Fcgr2b-cKO, and Notch2 Fcgr2b–dKO mice were immunized with NP-Ficoll. Serum and splenocytes were obtained 7 days later. (A and B) Representative examples of MZ B cell frequencies in Fcgr2b-cKO and Notch2 Fcgr2b–dKO mice. (C) Representative examples of intracellular IgG3 and NP staining in splenic PCs. (D) Frequency of NP-specific IgG3+ PCs in spleen, as a percentage of B cells. (E) Levels of NP-specific IgG3 in serum. (F) Frequency of NP-specific IgG3+ B cells in spleen. (G–I) Female control, Notch2-cKO, Fcgr2b-cKO, and Notch2 Fcgr2b–dKO mice were bred and maintained until 6–7 months of age, after which dsDNA antibodies in serum were characterized. dsDNA ELISAs for total IgM, IgG, and IgG subclasses were performed. Data are shown as the median, with each symbol representing an individual mouse (n = 5 mice per group for A–F; n = 13–17 mice per group for G–I). *P < 0.05, **P < 0.01, and ***P < 0.001, by 2-way ANOVA with Bonferroni’s post hoc test.