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. 2022 Sep 1;132(17):e153519. doi: 10.1172/JCI153519

Figure 8. ERN2 expression is associated with UC and goblet cell development in primary human colonoids.

Figure 8

(A and B) Left panels: scatter plots show relative mRNA expression for ERN2 in rectal biopsies from individuals with and without UC. Right panels: scatter plots show correlation between mRNA expression levels of ERN2 and goblet cell transcription factors ATOH1 or SPDEF in individuals with UC. (C) Representative images of undifferentiated and differentiated human colonoids. Fixed colonoids were stained with DAPI (blue) and phalloidin (magenta). Differentiated and undifferentiated colonoids were quantitated in cultures treated with DAPT and the IRE1 inhibitor 4μ8C. Symbols represent individual biological replicates from 3 independent experiments. Data are represented as mean ± SEM. Mean values were compared by 2-way ANOVA. Original magnification, ×10. (D) Bar graphs show relative mRNA expression for ATOH1, SPDEF, ERN2, and spliced XBP1 in primary human colonoids treated with DAPT and 4μ8C. Symbols represent individual biological replicates from 3 independent experiments. Data are represented as mean ± SEM. Mean values were compared by 2-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.