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MALDI-TOF analysis of free RNase B glycans in the culture supernatant after 2 h of E. faecalis BC002 growth. The culture supernatant was subjected to reverse-phase chromatography to remove hydrophobic contaminants and was desalted by ion exchange. Samples were analyzed using 2,5-dihydroxybenzoic acid as the matrix. ∗, molecular ions plus phosphate (98 mass units).
