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In vivo imaging of ARL13F53A-NG. Movie obtained by TIRF microscopy (left) and corresponding kymogram (right) showing ARL13F53A-NG in cilia of the arl13 mutant. IFT events are highlighted by exclamation marks in the corresponding frames of the movie and in the kymogram. A central part of the movie (∼50 s, see timer) was removed as during this period no IFT events were observed. The movie was recorded at 10 fps and the timer counts seconds. The movie is related to Fig. 4 and Fig. S4.
