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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Hepatology. 2021 Jun 21;74(3):1560–1577. doi: 10.1002/hep.31831

Fig. 5. The CD47-SIRPα signaling regulates the interaction between macrophage Gli1 and NICD in MSC-mediated immune regulation.

Fig. 5.

Bone marrow-derived macrophages (BMMs, 1x106) were co-cultured with MSCs or CD47-deficient MSCs (2x105) for 6h followed by LPS (100 ng/ml) stimulation. (A) (B) Western blot analysis and relative density ratio of CD47 and SIRPα in LPS-stimulated macrophages. (C) Analysis of mRNA levels and protein expression of Gli1 and NICD in macrophages after co-culture with MSCs or CD47-deficient MSCs by qRT-PCR and Western blot assay. (D) Immunofluorescence staining of nuclear Gli1 (red) and NICD (green) in macrophages after co-culture with MSCs or CD47-deficient MSCs. DAPI was used to visualize nuclei (blue). Scale bars, 20μm. (E) Immunofluorescence staining for macrophage Gli1 (red) and NICD (green) co-localization in the nucleus after co-culture with MSCs. DAPI was used to visualize nuclei (blue). Scale bars, 10μm. (F) Immunoprecipitation analysis of NICD and Gli1 in macrophages after co-culture with MSCs. All Western blots represent three experiments, and the data represent the mean±SD. *p<0.05, **p<0.01, ***p<0.001.