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mAb 28H4 was evolved to mAb hu6F15.4 with four sequential intermediates (28H4E10, 6F15, hu6F15.1, and hu6F15.3) from five designed single-chain variable fragment (scFv) libraries including two light chain shuffling libraries and three random mutation libraries. MAbs hu6F15.4 and hu6F15.6 showed cross-reactivity to seven BoNT/F subtypes.
