Table 2. Coordinates of interacting base pairs between (23660–23703 Spike) and (28025–28060 ORF8) for which nucleotide variations were observed.

Spike	ORF8	Power
23660	28060	0
23661	28059	0
23662	28058	0
23663	28057	0.08
23664	28056	0.11
23671	28050	0
23672	28049	0
23673	28048	0.39
23674	28046	0
23675	28045	0.05
23676	28044	0.04
23677	28043	0
23679	28042	0
23680	28041	0.01
23681	28040	0
23682	28039	0
23683	28038	0
23684	28037	0
23685	28036	0
23686	28035	0
23687	28034	0
23688	28033	0.01
23689	28032	0
23690	28031	0

Total number of sequences was 206,745. Column power is an output of the R-scape software that is proportional to the statistical power of substitutions. Mutations in coordinates in black bold are shown Fig 3. Base pairs within the Core interacting region (Fig 2) are shown in cells shaded red.