Table 1. Quantification of plaque assay for the four T. gondii strains.

s.d.: Standard Deviation. Cytolytic efficiency (CE) = (Total area of the host cell monolayer lysed by a parasite line)/(Total area lysed by the WT parasite). P-values from unpaired Student’s t-tests are indicated on the right. P_n: P-values for comparison of number of plaques. P_a: P-values for comparison of average area of plaques.

Strain	Number of plaques± s.d.	average area (mm2)± s.d.	CE (%WT)	RHΔhxΔku80 (WT)	mEmeraldFP-Pcr2 knock-in	Δpcr2	complement
RHΔhxΔku80 (WT)	41±3	0.96±0.15	100		pn = 0.039	pn = 0.0002	pn = 0.239
					pa = 0.747	pa = 0.015	pa = 0.649
mEmeraldFP-Pcr2 knock-in	35±2	1.02±0.24	89.7			pn = 0.0008	pn = 0.658
						pa = 0.034	pa = 0.869
Δpcr2	9±3	0.31±0.03	7.5				pn = 0.049
							pa = 0.046
complement	32±10	1.05±0.29	89.1