FIGURE 4.

Full breakdown of telomere length distribution for fibroblasts treated with TriHex™ for six weeks. Human primary neonatal fibroblasts were treated with the TriHex™ at 100, 50, and 3 μg/mL for six weeks. The TAT assay was conducted to determine the telomere lengths. A) The telomere length percentiles are one hundred individual telomere length measurements per sample, including the shortest telomeres' length (1st percentile length) and the length of the longest telomeres (100th percentile length). The percentiles allow for a comprehensive comparison between all the telomere lengths present in each sample throughout the telomere length distribution. B) The overall short telomere length variables are the percentages of telomeres with a specific telomere length. The variables identify whether one sample has a higher percentage (quantity) of shorter or longer telomeres and detect differences between samples in a specific area of the distribution. C) The assessment of cells with short telomere variables are the percentages of cells with a specific average telomere length. Since senescence and cell apoptosis affect the cells, this value addresses the telomeric profile on a cellular level. This value detected whether a sample presents a higher percentage of cells with shorter telomeres (closer to senescence) or longer ones.