Fig. 6.

Immunization with 10⁴ pfu of rVSV-preS-HexaPro and rVSV-preS-2P vaccines provides complete protection against SARS-CoV-2 challenge. (A) Dynamics of mouse weight changes. The body weight was expressed as percentage of body weight at the challenge day. The average body weight of 5 mice (n = 5) in each group was shown. SARS-CoV-2 titer in lungs (B) and nasal turbinate (C). At day 4 after challenge, all mice were killed, and lungs and nasal turbinates were collected for virus titration by plaque assay. Viral titers are the GMT of 5 animals ± SD. The limit of detection (LoD) is 2.91–2.99 Log₁₀ pfu per gram of tissue (dotted line). Data were analyzed using one-way ANOVA. (D) Lung pathology score after challenge with MA SARS-CoV-2. Each lung slide was quantified based on the severity of histologic changes. Score 4 = extremely severe lung pathological changes; score 3 = severe lung pathological changes; score 2 = moderate lung pathological changes; score 1 = mild lung pathological changes; and score 0 = no pathological changes.