FIG. 3.

Western immunoblot detection of GldB and GldC in wild-type and mutant strains of F. johnsoniae. Cells were lysed in SDS-PAGE loading buffer, and proteins were separated by SDS-PAGE and transferred to PVDF membranes. GldB (A) and GldC (B) were detected using antisera raised against recombinant GldB or GldC proteins, respectively, as described in Materials and Methods. Lanes: 1, wild-type F. johnsoniae; 2, gldB insertional knockout mutant CJ588; 3, CJ767 (CJ588 complemented with pDH246, which carries gldB); 4, CJ594 (CJ588 complemented with pDH233, which carries gldB and gldC); 5, Tn4351-induced gldB mutant CJ562; 6, Tn4351-induced gldB mutant CJ567.