TABLE 1.
Plasmids used in this studya
| Plasmid | Description | Source or reference |
|---|---|---|
| pBC SK(+) | ColE1 ori; Cmr | Stratagene |
| pCR-Script Amp SK(+) | ColE1 ori; Apr | Stratagene |
| pMAL-c2 | ColE1 ori; Apr; malE fusion expression vector | New England BioLabs |
| pBAD/His-C | ColE1 ori; Apr; His-tag expression vector | Invitrogen |
| pNJR6 | RSF1010 ori; Knr (Emr) | 44 |
| R702 | IncP; Kmr Smr Tcr; helper plasmid for triparental conjugation | 18 |
| pLYL03 | ColE1 ori; Apr (Emr); Bacteroides-Flavobacterium suicide vector used to make chromosomal insertions | 25 |
| pTGL130 | ColE1 ori; Apr Tcr | 11 |
| R751::Tn4351Ω4 | IncP; Tpr Tcr (Emr); Vector used for Tn4351 mutagenesis | 39 |
| pEP4351 | pir-requiring R6K oriV; RP4 oriT; Cmr Tcr (Emr); vector used for Tn4351 mutagenesis | 9 |
| pCP11 | ColE1 ori; (pCP1 ori); Apr (Emr); E. coli-F. johnsoniae shuttle plasmid | 27 |
| pCP19 | (pCP1 ori); (Emr); F. johnsoniae plasmid | 2 |
| pCP22 | RSF1010 ori; (pCP1 ori); Knr (Emr); E. coli-F. johnsoniae shuttle cosmid | This study |
| pCP23 | ColE1 ori; (pCP1 ori); Apr (Tcr); E. coli-F. johnsoniae shuttle plasmid | 2 |
| pCP26 | RSF1010 ori; (pCP1 ori); Knr Tcr (Emr); E. coli-F. johnsoniae shuttle cosmid | This study |
| pCP200 | RSF1010 ori; (pCP1 ori); Tcr (Emr); cosmid clone complementing F. johnsoniae UW102-99 | This study |
| pDH65 | Nucleotides 145–754 of gldB in pBC SK(+); Cmr | This study |
| pDH222 | 1.9-kb XbaI-ClaI fragment of pCP200 in pBC SK(+); Cmr | This study |
| pDH223 | 1.9-kb XbaI-SalI fragment of pDH222 in pCP11; Apr (Emr) | This study |
| pDH226 | 850-bp PstI-PvuII fragment of pDH65 cloned into PstI-SmaI-cut pBC SK(+); Cmr | This study |
| pDH227 | 854-bp PstI-BamHI fragment of pDH226 cloned into pLYL03; Apr (Emr); plasmid for gldB gene disruption | This study |
| pDH233 | 1.9-kb XbaI-KpnI fragment of pDH222 in pCP23; Apr (Tcr) | This study |
| pDH238 | 497-bp fragment containing 3′ end of gldB in SrfI site of pCR-Script SK(+); Apr | This study |
| pDH240 | 368-bp fragment containing gldC in SrfI site of pCR-Script SK(+); Apr | This study |
| pDH241 | 335-bp PstI-HindIII fragment of pDH240 in pBAD/His-C; Apr; plasmid for gldC overexpression | This study |
| pDH242 | 1-kb fragment containing gldB in SrfI site of pCR-Script SK(+); Apr | This study |
| pDH243 | 1-kb fragment containing gldB in pCP11; Apr (Emr) | This study |
| pDH245 | 1-kb fragment containing gldB in pBC SK(+); Cmr | This study |
| pDH246 | 1-kb fragment containing gldB in pCP23; Apr (Tcr) | This study |
| pDH250 | 479-bp PstI-HindIII fragment of pDH238 in pMAL-c2; Apr; plasmid for gldB overexpression | This study |
a
Antibiotic resistance phenotypes: ampicillin, Apr; chloramphenicol, Cmr; erythromycin, Emr; kanamycin, Knr; streptomycin, Smr; tetracycline, Tcr; trimethoprim, Tpr. Unless indicated otherwise, antibiotic resistance genes and origins of replication are those expressed by E. coli. Antibiotic resistance phenotypes and other features listed in parentheses are those expressed by F. johnsoniae but not by E. coli.