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. 2022 Aug 18;13:952852. doi: 10.3389/fimmu.2022.952852

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Copyright © 2022 Ren, Ding, He, Wei, Shi and Deng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SeMet inhibits the infectivity of PDCoV. (A) LLC-PK1 were infected with PDCoV (100 TCID_{50 =} 10^-2.15) for 1.5 h and treated with 0~150 μM of SeMet. Cells were collected after 48 h for qRT-PCR of viral M gene (n=6). (B) At 48 h treatment with SeMet, PDCoV (100 TCID₅₀) replication in LLC-PK1 cells was determined by indirect immunofluorescence assay (IFA). (C) LLC-PK1 cells were infected with PDCoV for 1.5 h and treated with SeMet (150 μM) for 6, 24, and 48 (h) Expression level of viral mRNAs was analyzed by qRT-PCR (n=6). (D) At 6~48 h treatment with SeMet, PDCoV replication in LLC-PK1 cells was determined by IFA. Means ± SD are shown. Statistical significance was determined by Student t test. ***, P<0.001; **, P<0.01; *, n.s, not significant. All experiments were repeated at least twice and representative results are shown.