Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 18;13:952852. doi: 10.3389/fimmu.2022.952852

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Ren, Ding, He, Wei, Shi and Deng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Decreased STAT3 levels are utilized by SeMet to suppress PDCoV replication. (A) Expression level of viral mRNAs was analyzed by qRT-PCR when transfected with pcDNA3.1-STAT3, empty vector, siSTAT3, siRNA-NC, DMSO, Stattic (0.75 μM) (n=6). (B) Cells were transfected with pcDNA3.1-vector or pcDNA3.1-STAT3, followed by treatment with SeMet and PDCoV. Viral mRNA expression was analyzed by qRT-PCR (n=6). (C) Expression level of viral mRNAs was analyzed by qRT-PCR when transfected with siSTAT3 and miR-125b-5p-1 inhibitor followed by infection with PDCoV (n=6). Means ± SD are shown. Statistical significiance was determined by Student t test. ***, P<0.001; **, P<0.01; *, P<0.05; n.s, not significant. All experiments were repeated at least twice and representative results are shown.