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Upmodulation of ACSL4 enhances ferroptosis. (a) mRNA expression of ACSL4 after transfection in specified cells. (b, c) In A549 and SPC-A-1 cells, upregulation of ACSL4 enhanced erastin-induced cell death. (n = 3, ∗∗∗p < 0.001 vs. control cDNA group). (d, e) After 24-hour treatment with erastin, lipid ROS and iron in A549 and SPC-A-1 cells were measured (n = 3, ∗∗p < 0.01 vs. control cDNA subgroup).
