(a).

Culture medium/supplement/culture strategy	Regions/organoids type	Key features	References
SHH, FGF8	Midbrain simBOs	High efficiency, high homogeneity, easy to specify	[58]
CHIR99021	Midbrain-like MLOs	Robust generation Homogenous distribution of mDAs, other neuronal subtypes, and functional glial cells, such as astrocytes and oligodendryocytes	[61]
WNT3A and mixed medium with 1 : 1 of fresh and supernatant derived from interfollicular epidermal SCs	Epidermal organoids	Functional with polarity	[120]
RSPO1, WNT3A, WNT7A	Endometrial organoids	Endometrial disease facilitate growth of endometrial disease organoids and the long-term expansion	[121]
WNT and nodal antagonists	Hippal/CB organoids	Original differentiation method Low efficiency of O2 and nutrient diffusion	[122]
Dkk1 and LeftyA
Floating culture with 40% O2 and 5% CO2 CHIR 99021, BMP4	Hipp/cortex organoids		[10, 13, 24,119–121]