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. 2022 Aug 25;2022:3096528. doi: 10.1155/2022/3096528

Figure 3.

Figure 3

Severe asthma was mediated by Th17 cells (IL-17) and the differentiation and expression of Th2/Th17 cells (IL-4/IL-17) under the influence of DHT, E2, and DHT/E2. (a) Detection and measurement of Th2 and Th17 cells by flow cytometry from the splenocytes. The severe asthma group showed an increase in Th17 cells than asthma and the normal control group, whereas the severe asthma+E2 group showed a much more obvious increase in Th17 cells than the severe asthma+ DHT and severe asthma+ DHT/E2 groups. The percentage of Th2 and Th17 cells was also shown, and the results correlated with flow cytometric analysis. (b) Concentration of IL-4 and IL-17 in different groups from splenocytes by ELISA. Higher IL-17 concentration was observed in the severe asthma group than in asthma and normal control. Severe asthma+E2 showed an obvious increase in IL-17, while the severe asthma+ DHT significantly reduced the IL-17 showing the therapeutic potential. (∗p < 0.05; ns: not significant).