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. 2022 Aug 25;2022:3096528. doi: 10.1155/2022/3096528

Figure 5.

Figure 5

Detection and expression of GATA3 and RORγt in severe asthma and influence of DHT, E2, and DHT/E2 on GATA3 and RORγt expression. After the identification of Th17 cells as the kernel of severe asthma (Figures 3 and 4), we measured the expression of GATA3 and RORγt in animal and cellular models of severe asthma. (a) Lung WB detection of GATA3 and RORγt and relative protein expression in normal control, asthma, and severe asthma mice model. A significant level of RORγt expression was noted in severe asthma than in the other groups correlating with the higher Th17 cells expression (Figure 3) in severe asthma. (b) Lung WB detection of GATA3 and RORγt and relative protein expression in mice model with DHT, E2, and DHT/E2 influence. We observed a significant decrease in RORγt detection and expression after the addition of DHT in the severe asthma +DHT group as compared to severe asthma, severe asthma+E2, and severe asthma+ DHT/E2 group. (c) After the detection of BECs and measurement of IL-4 and IL-17 (Figures 4(a) and 4(b)), WB detection of GATA3 and RORγt and relative protein expression was performed in the BECs model. Th17 cells predominant BECs severe asthma model was induced by treating BECs with 100 μg/ml HDM and 100 ng/ml LPS, 100 μg/ml HDM to induce T2 asthma (asthma), and PBS for normal controls. Severe asthma was mediated by a significant higher level of RORγt expression than the normal control and asthma group. (d) Androgen in severe asthma+ DHT decreased the RORγt detection but significantly higher RORγt detected in severe asthma+ E2 group and the RORγt detection in severe asthma+ DHT/E2 was in between the independent effect of DHT and E2 alone. The relative protein expression results were similar to WB results (∗p < 0.05, ∗∗p < 0.01).