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. 2022 Aug 25;2022:3096528. doi: 10.1155/2022/3096528

Figure 7.

Figure 7

Effects of DHT, E2, and DHT/E2 on MBD2 detection and expression under MBD2 silencing or overexpression (OE) in BECs severe asthma model. (a) The expression of MBD2 at its silencing under the influence of DHT, E2, and DHT/E2 has been shown. Severe asthma was mediated by increased MBD2 expression, and as obvious, SiR-MBD2 reduced the MBD2 which was cross-checked by SiR-NC-MBD2. Severe asthma + DHT reduced the expression of MBD2 as compared with severe asthma, severe asthma +E2, and severe asthma +DHT/E2 group showing the androgenic therapeutic potential. Similarly, reduced MBD2 detection was noted with silencing in severe asthma +DHT (SiR-MBD2+DHT) as compared with SiR-MBD2 in severe asthma. Severe asthma + E2 was associated with increased MBD2 as compared with the severe asthma group and silencing of MBD2 decreased its detection, while SiR-NC-MBD2 was associated with significantly increased MBD2 expression. SiR-MBD2+E2 showed comparatively higher MBD2 detection than the SiR-MBD2+DHT. The severe asthma+ DHT/E2 group was observed with reduced MBD2 detection better than severe asthma+ E2, but not to that level of severe asthma+ DHT. Expression of MBD2 protein at its silencing also has been shown and corresponded with WB results. (b) The expression of MBD2 at its OE under the influence of DHT, E2, and DHT/E2. Severe asthma was associated with increased MBD2 detection than the normal control, and OE-MBD2 increased its detection, while as usual, it was counter-checked by OE-NC-MBD2. Androgen in (severe asthma + DHT) reduced the MBD2 detection, and the OE-MBD2+DHT group increased the MBD2 detection, but the detection was lowest than all the OE groups showing the androgenic therapeutic potential. Androgen estrogen ratio in severe asthma+ DHT/E2 did not reduce the MBD2 expression as severe asthma+ DHT. The expression of MBD2 protein at its OE was also shown in the figure. #Severe asthma vs severe asthma+ DHT, severe asthma+E2, and severe asthma+ DHT/E2. ##Severe asthma vs severe asthma+E2 and & severe asthma+ DHT vs severe asthma+ DHT/E2 (∗p < 0.05, ∗∗p < 0.01, #p < 0.05, ##p < 0.01, &p < 0.05).