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. 2022 Aug 25;2022:3096528. doi: 10.1155/2022/3096528

Figure 9.

Figure 9

Effects of DHT, E2, and DHT/E2 on detection and expression of RORγt and GATA3 under MBD2 silencing or OE in BECs severe asthma model. (a) We detected the expression of GATA3 and RORγt at MBD2 silencing under the influence of DHT, E2, and DHT/E2 by WB. Comparatively, higher RORγt is detected than the GATA 3 in the severe asthma group coinciding with the higher Th17 (IL-17) cells as shown in Figures 8(a), 8(c), and 8(d). Silencing of MBD2 decreased the detection of RORγt in severe asthma significantly, but not GATA3 which was counter confirmed by SiR-NC-MBD2. The addition of androgen in the severe asthma+ DHT group significantly reduced RORγt detection than the GATA3 as compared with severe asthma. SiR-NC-MBD2 and SiR-MBD2 group of DHT showed significant changes in RORγt detection, but not GATA3 showing androgenic potential in decreasing RORγt via MBD2. Estrogen in severe asthma+E2 significantly increased the RORγt detection, while silencing of MBD2 decreased the RORγt detection, but not GATA3. Androgen estrogen in severe asthma+ DHT/E2 decreased RORγt detection than severe asthma and severe asthma+E2, but not to the level of alone as in severe asthma+ DHT group showing the most potent effect of DHT in RORγt detection regulating MBD2. (b) WB detection of GATA3 and RORγt at MBD2 OE under the influence of DHT, E2, and DHT/E2. Severe asthma was mediated by higher RORγt, and OE-MBD2 persistently showed higher RORγt detection as confirmed by OE-NC-MBD2. OE-MBD2 in the androgen group (OE-MBD2+ DHT) increased RORγt detection; however, the detection was lower than the OE in severe asthma (OE-MBD2) showing androgenic potential, and as expected, OE-NC-MBD2+ DHT decreased the detection. Interestingly, OE-MBD2+DHT and OE-NC-MBD2+DHT did not significantly change the detection of GATA3 which also showed the potential role of DHT on RORγt expression. OE-MBD2 in the estrogen group (OE-MBD2+E2) was associated with a significant increase in RORγt than without OE in severe asthma+E2. Expression of RORγt and GATA3 protein under MBD2 silencing or OE with the effect of DHT, E2, and DHT/E2 correlated with WB detection (c, d, e, f). #Severe asthma vs severe asthma+ DHT, severe asthma+E2, and severe asthma+ DHT/E2 and & severe asthma+ DHT vs severe asthma+ DHT/E2. (∗p < 0.05, #p < 0.05, ##p < 0.01, &p < 0.05, and ns: not significant).