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. 2022 Aug 25;2022:2527210. doi: 10.1155/2022/2527210

Figure 2.

Figure 2

Cold induces CIRBP expression to affect the expression of key regulatory factors of ferroptosis. (a) Searching for factors that interact with CIRBP in the GeneCards database. (b) RIP showed that CIRBP antibody significantly enriched p53 RNA. ∗P < 0.05 versus IgG; #P < 0.05 versus CIRBP. (c) Agarose gel electrophoresis showed the expression of p53. BxPC-3 and PANC-1 cells were cultured at 37 (Control) or 32°C, then the expression of CIRBP (d) and p53 (e) was detected by qRT-PCR, and western blot analysis (f) was used to detect the protein expression levels of CIRBP (g), p53 (h), DPP4 (i), GPX4 (j), NOX1 (k), and FTH1 (l). ∗P < 0.05 versus Control-BxPC-3; #P < 0.05 versus Control-PANC-1.