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Cold induces Fe2+ accumulation and abnormal oxidative stress. BxPC-3 and PANC-1 cells were cultured at 37 (Control) or 32°C. (a) The increase in Prussian blue staining indicated an increase in Fe2+ accumulation level. (b) Representative confocal fluorescent images of MitoTracker™ Red FM (the mitochondrial indicator) and DCFH-DA (ROS indicator)-loaded cells. (c) GSH-Px activity was measured using a GSH-Px assay kit. ∗P < 0.05 versus Control.
