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Cold induces ferroptosis to regulate the proliferation and apoptosis of pancreatic cancer cells. BxPC-3 and PANC-1 cells were cultured at 37°C (Control), 32°C, or treated with ferroptosis inhibitor Ferrostatin-1 (Fer-1) and then cultured at 32°C (32°C+Fer-1). The proliferation of BxPC-3 (a) and PANC-1 (b) cells was detected by CCK-8 assay. (c–e) Detection of apoptosis by flow cytometry. ∗P < 0.05 versus Control.
