Figure 7.

Myocardial ODC1-PUT Metabolic Flux Is Required for STING-NFκB Pathway–Driven Cardiac Hypertrophic Program in a CKD Milieu

(A to C) Relative mRNA levels of Odc1(left) and the contents of PUT (right) in NRMCs incubated with normal or CKD serum after sip65 (A), sicGAS (B), or siSting (C) treatment. (D) Binding of p65 on Odc1 promoter was assayed using chromatin immunoprecipitation. (E) Relative mRNA levels of ANF, BNP, and β-MHC in normal and CKD serum–incubated NRCMs with siOdc1 pretreatment and PUT supplementation. (F to H) Relative mRNA levels of ANF, BNP, and β-MHC in normal and CKD serum–incubated NRCMs with sicGAS (F), siSting (G), or sip65 (H) pretreatment and PUT supplementation. Data are represented as mean ± SD and were analyzed using 1-way analysis of variance. n = 3 biologically independent experiments. n∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. Abbreviations as in Figures 1, 2, and 6.