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Representative surface plasmon resonance (SPR) sensorgrams of CD16a allotypes. Glycoforms are complex-types (A and E) with two variants missing the N162 glycan (C and G). Fits of the dissociation constants and associated errors from equilibrium binding measurements are also shown (B, D, F, and H). Cartoons show the expected N-glycan at the N162 site. The S164A mutation disrupts the N162 N-glycan sequon and thus lacks an N-glycan at that site.
