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. 2022 Aug 1;298(9):102333. doi: 10.1016/j.jbc.2022.102333

Figure 3.

Figure 3

SR-BI aspartic acid mutants express in whole-cell lysates. A, immunoblot analysis using a C-terminal SR-BI targeting antibody was performed on whole-cell cleared lysates (10 μg) from COS-7 cells transiently expressing empty vector, WT-SR-BI, or individual aspartic acid mutants. GAPDH expression was probed as a loading control. Each blot is representative of three independent transfections (n = 3). B, densitometry of immunoblots was performed using NIH ImageJ software, and quantification of SR-BI expression relative to loading control is shown (WT-SR-BI = 100%). SR-BI, scavenger receptor class B type I; WT, wildtype.