Skip to main content
. 2022 Aug 1;298(9):102333. doi: 10.1016/j.jbc.2022.102333

Figure 4.

Figure 4

SR-BI aspartic acid mutants express at the cell surface. Cell surface biotinylation was performed on COS-7 cells transiently expressing empty vector, WT, or mutant SR-BI receptors. Biotinylated proteins (10 μl lysates, protein concentration [mean ± SD] = 9.4 μg ± 2.5 μg) were immunoblotted for SR-BI expression using a C-terminal SR-BI targeting antibody. A 10-μL aliquot of each total lysate, prior to biotin pull-down, was also immunoblotted for SR-BI (see “total proteins”, to account for differences in overall SR-BI expression) and GAPDH (to account for differences in the protein amounts incubated with streptavidin beads). Each blot is representative of three independent transfections (n = 3). SR-BI, scavenger receptor class B type I; WT, wildtype.