Figure 5.

SR-BI aspartic acid mutants retain the ability to dimerize or oligomerize. COS-7 cells transiently expressing empty vector, WT, or aspartic acid SR-BI mutants were harvested in ice-cold PBS and lysed by sonication. Lysates (5 or 10 μg) were incubated in sample buffer containing PFOA (final concentration = 2.5%) and separated on 8% PFO-PAGE gels. Immunoblotting analysis using the C-terminal SR-BI targeting antibody was performed to assess the oligomerization profiles of SR-BI. Blots are each representative of three independent transfections (n = 3). SR-BI monomers (M), dimers (D), and higher-order oligomers (HO) are indicated. Note that hydrophilic mutants are not in numerical order. PFOA, perfluorooctanoic acid; SR-BI, scavenger receptor class B type I; WT, wildtype.