Figure 2.

Ess2^ΔCD4/ΔCD4mice show aberrant T-cell development in splenocytes.A, representative spleens (left panel), quantification of splenocytes (middle panel), and spleen weights (right panel) in Ess2^fl/fl and Ess2^ΔCD4/ΔCD4 mice. Scale bar = 1 mm. For flow cytometry, the following experiments were carried out under the same conditions: panels B and C; panels D–F; and panels G–I. B, representative population of B and T cells among the splenocytes of Ess2^fl/fl and Ess2^ΔCD4/ΔCD4 mice as measured by flow cytometry. C, quantification of B cells and T cells in Ess2^fl/fl and Ess2^ΔCD4/ΔCD4 mice. D, a representative population of CD4⁺ or CD8⁺ T cells as assessed by flow cytometry. E, quantification of CD4⁺ T cells in splenocytes and total CD3⁺ T cells. F, quantification of CD8⁺ T cells in splenocytes and total CD3⁺ T cells from. G, a representative population of naïve and memory CD4⁺ T cells as assessed by flow cytometry. H, quantification of naïve CD4⁺ T cells in total splenocytes, total CD3⁺ T cells, and total CD4⁺ T cells from splenocytes. I, quantification of memory CD4⁺ T cells in total splenocytes, total CD3⁺ T cells, and total CD4⁺ T cells from splenocytes. J, a representative population of γδT cells as assessed by flow cytometry (left and middle panels) and the quantification of γδT cells in splenocytes (right panel). K, a representative population of NKT cells as assessed by flow cytometry (left and middle panels) and the quantification of NKT cells in splenocytes (right panel). L, RT-qPCR analysis of primary CD4⁺ T cells cultured under T_H17 conditions. The mRNA levels of all genes were normalized to the level of Gapdh mRNA expression. Each experiment was performed at least three times and the results are presented as the mean ± SD. Each experiment used 3 to 7 mice. p values were calculated using Student’s t test, ∗p < 0.05. qPCR, quantitative polymerase chain reaction.