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. 2022 Sep 1;12:14902. doi: 10.1038/s41598-022-19065-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-Seq revealed that 7-KC regulated the expression of transcripts related to inflammation, cholesterol biosynthesis and ER stress. RNA-Seq was performed using RNAs extracted from peritoneal macrophages treated with vehicle, 10 µM cholesterol or 7-KC for 24 h. N = 3. (A) Transcriptomic heatmap for the 3 groups. The heatmap was visualized using iDEP software (version 0.94) (http://bioinformatics.sdstate.edu/idep/). (B) and (C) Network analysis (B) and top 30 differentially overexpressed mRNAs (C) in macrophages treated with 7-KC compared to those treated with cholesterol. Transcripts were categorized as inflammation- (red), cholesterol/steroid metabolism- (green), and ER stress-associated genes (blue). The network analysis was visualized using STRING software (version 11.5) (https://string-db.org). RNA-Seq, RNA sequencing; Veh, vehicle; Chol, cholesterol; 7-KC, 7-ketocholesterol; ER, endoplasmic reticulum; FC, fold change.