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. 2022 Sep 1;13(9):754. doi: 10.1038/s41419-022-05182-0

Fig. 2. Ivermectin led to G0/G1 arrest, apoptosis, and DNA damage in prostate cancer.

Fig. 2

A The ivermectin arrest cell cycle at G0/G1 was measured by flow cytometry. LNCaP, C4-2, and 22RV1 cells were treated with ivermectin at 4, 8, and 12 μM for 48 h. B Ivermectin induced cell apoptosis detected by PI/Annexin V staining. Cells were treated as in A. The PI + /Annexin V + and PI-/Annexin V + cells were calculated as apoptotic cells. C Western blot analysis of PARP and cleaved-Caspase-3 (c-Caspase-3) in cells treated with ivermectin for 48 h. D Ivermectin increased DNA damage. DNA fragments were shown as comet images in alkaline gel electrophoresis. The tail moment was used to quantify the DNA damage in the treatment of ivermectin for 48 h. E Western blot analysis of γH2A.X in cells treated with the ivermectin for 48 h. F Tumor volume of 22RV1 xenografts after castration treated with vehicle (con) or ivermectin (10 mg/kg, n = 5 for each group). G Representative images of Ki67, γH2A.X, and PSA immunostaining, in 22RV1 tumors treated with vehicle or ivermectin.