Fig. 2. TSLP is differentially implicated for e.c. and d.c. HDM sensitization-induced asthmatic inflammation.
a Total cell number in bronchoalveolar (BAL) fluid. NS, non-sensitized. b Cell number of eosinophils, neutrophils, lymphocytes and macrophages in BAL fluid. For a, b, n = 2, 3, 5, 5, 2, 4, 4 mice. c Quantitative RT-PCR analyses of BAL cells (n = 2, 3, 3, 3, 2, 4, 4 mice). d Lung paraffin sections were stained with hematoxylin & eosin (H&E), analysed by immunohistochemistry (IHC) staining with anti-MBP antibody (for eosinophils) or MCPT8 antibody (for basophils), or by Periodic Acid Schiff (PAS) staining for mucus-producing goblet cells (stained as purple). B: bronchiole. V: blood vessel. Black arrows point to one of the positive cells. Bar = 50μm for all pictures. e Lung resistance (RL) at the baseline (aerosol of PBS) and in response to aerosolized methacholine (Mch; 50 mg/ml), measured by FlexiVent system (n = 8, 8, 4, 10, 3 mice). Graphs in a–c, e show mean ± SEM. Two-sided Student’s t-test. Data are representative of 3 (a–d) or 2 (e) independent experiments with similar results. Source data are provided as a Source Data file.