Fig. 7. Administration of IL-1β restores the d.c. HDM-induced asthmatic phenotype in NIMP-R14-treated mice.
a Experimental protocol. Wildtype Balb/c mice were intraperitoneally (i.p.) injected with PBS or NIMP-R14 antibody at day (D) -1 and D2. Mice were d.c. sensitized with HDM ± IL-1β on LMP_91μm ears at D0 and D3. All mice were intranasally (i.n.) challenged with HDM from D9 to D12 and analysed at D13. b Total cell number and differential cell counting in BAL fluid (n = 5, 4, 5 mice). One-way ANOVA test. c Relative RNA levels of genes in BAL cells (n = 5, 4, 4 mice). One-way ANOVA test. d Lung sections were stained with hematoxylin-eosin (H&E) or Periodic Acid Schiff (PAS). B: bronchiole. V: blood vessel. Bar = 250 μm for all pictures. e Lung resistance (RL) at the baseline (aerosol of PBS) and in response to aerosolized methacholine (Mch; 50 mg/ml), measured by FlexiVent system (n = 4, 5, 4, 5 mice). Two-sided Student’s t test. f Serum level of HDM-specific IgE and IgG1 measured by ELISA (n = 5, 4, 5 mice). Two-sided Mann–Whitney rank sum test. NS, non-sensitized. Data are representative of 2 (b–d, f) or 1 (e) independent experiments with similar results. Source data are provided as a Source Data file.