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. 2022 Sep 1;12:14833. doi: 10.1038/s41598-022-19103-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the expression of genes involved in biofilm formation of MRSA after treatment with different concentrations of rhamnolipid (15, 30, 60 mg/mL) by using real-time PCR technique; the expression of all studied genes such as; (a) agrA, (b) agrC, (c) icaA, (d) icaD, and (e) srtA compared with their untreated conditions (with neutral pH) had a significant decrease. There is a statistically significant difference between groups that showed by the symbol *P < 0.05 and **P < 0.005 via one-way ANOVA (Tukey test) and Changes in expressing all columns of each diagram are drawn compared to control mode.