Table 3:
Overview of CpGs, which are present in more than one of the best performing MRSs and their association with allergy-related outcomes in the original EWAS (A) and in the LISA cohort (replication, B).
| CpG (gene) |
Allergy-related outcome |
cg04983687 (ZFPM1) |
cg09249800 (ACOT7) |
cg11699125 (ACOT7) |
cg11988722 (intergenic) |
cg12077460 (MFHAS1) |
cg14011077 (intergenic) |
cg17971251 (SEC16B) |
|---|---|---|---|---|---|---|---|---|
| A. Effect direction (p-values 1 ) from original EWAS | ||||||||
| Everson20156 | Atopy | − (6.46E-09) | − (8.52E-09) | |||||
| Chen20177 | IgE | − (1.50E-12) | − (2.60E-11) | − (6.70E-07) | ||||
| Peng20198 | Aeroallergens | − (7.11E-15) | − (7.28E-12) | − (6.51E-14) | − (4.03E-11) | |||
| Zhang20199 | Atopy | − (1.17E-04) | − (4.34E-05) | − (9.04E-05) | − (2.98E-05) | − (2.83E-05) | − (1.46E-05) | |
| Reese201910 | Asthma | − (1.33E-10) | − (1.19E-08) | − (7.54E-10) | − (7.02E-09) | − (9.52E-09) | ||
| Xu 202111 | Any allergy | − (5.84E-19) | − (3.73E-13) | |||||
| B. Beta coefficients (p-values 2 ) in LISA | ||||||||
| LISA: 6 years | Aeroallergen sensitization | −11.61* (1.62E-03) |
−14.71* (1.53E-02) |
−11.94* (3.14E-02) |
−21.59* (6.31E-04) |
−19.65* (3.17E-03) |
−40.89* (1.02E-02) |
−26.84* (5.19E-04) |
| LISA: 10 years | Aeroallergen sensitization | −3.93 (1.62E-01) |
−9.77* (2.79E-03) |
−8.41* (2.47E-03) |
−10.04* (2.35E-02) |
−6.61 (4.27E-01) |
−14.67 (1.22E-01) |
−12.90* (8.01E-03) |
In A, effect direction (+/−) is reported instead of effect estimates because of the different allergy-related outcomes that were used in the original EWAS, which do not allow a direct comparison of effect estimates.
2
raw p-values given corrected for Houseman cell type estimates
*
These CpGs were all successfully replicated in the LISA cohort (FDR threshold of 0.05)