Fig. 6.

In vitro fertilization with cumulus-intact and ZP-free oocytes. a Microscopic image of the fertilized oocytes at zygotes or 2-cell stage. There was no observation of fertilized oocytes incubation with the sperms of C11orf94^−/− male mice. Scale bar, 200 μm. b-c High-resolution picture of oocytes after incubated with the sperms of C11orf94^+/+ or C11orf94^−/− male mice. Asterisks indicate male and female pronucleus and arrows indicate sperms gathered in perivitelline space. Scale bar, 100 μm. d-e The ratio of zygotes or 2-cell development to total oocytes. There was no significant difference between cumulus-intact and ZP-free groups