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. 2022 Sep 2;3:27. doi: 10.1186/s43556-022-00092-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Analysis of protein changes in testis and sperm of C11orf94-deficient mice by mass spectrometry. a Venn diagram analysis of differential genes between testis and sperm. There are 1070 differential genes in testis and 2425 differential genes in sperm between C11orf94^+/+ and C11orf94^−/− mice. b Go analysis of differentially expressed overlapping proteins in testis and sperm. c-d Analysis of differential genes related to Ca.²⁺ signalling pathway (c) and sperm-oocyte fusion (d) in mature spermatozoa (n = 3) (*, p < 0.05; **, p < 0.01; ***, p < 0.005)