FIGURE 6.

DEX promoted the polarization of microglia from the M1 to M2-like phenotype in neonatal rats with HIBD. Brain sections from the ipsilateral hemisphere on D2 were stained for anti-IBA-1 antibody (green) and (A) for anti-IL-1β, a marker for the M1 phenotype (red); and (C) for anti-Arg-1, a marker for the M2 phenotype (red). (A–D) Representative IF images (magnification ×400, scale bar: 50 μm) and semi-quantitative analysis (bar chart) of the ratios of IBA-1⁺\ IL-1β⁺ vs. total IBA-1⁺ and IBA-1⁺\ Arg-1⁺ vs. total IBA-1⁺ showing the status of microglia polarization. DEX, Dexmedetomidine; HIBD, Hypoxic-ischemic brain damage; D2, 2 days after HIBD. Data are expressed as the mean ± SD (n = 5 per group). *p < 0.05 vs. the HI group.