FIGURE 3.

FG‐4592 inhibits apoptosis in bone marrow stromal cell (BMSC) post‐oxygen–glucose deprivation (OGD) through activating HIF‐1α/BNIP3‐dependent autophagy. (A, B) Apoptosis measured by flow cytometry (FCM), and statistics were displayed as mean ± SD. (C–E) Western blot images and relative quantification of apoptosis‐related proteins (Bcl‐2, Bax) and Gapdh (n = 4). (F–K) Expression of HIF‐1α, BNIP3, P62, Beclin‐1, LC3, and Gapdh in BMSCs was detected by western blot. And relative quantification of HIF‐1α, BNIP3, P62, Beclin‐1, LC3 (n = 4). * means p value <0.05, ** means p value <0.01, and *** means p value <0.001; n.s. means no significance, p values were calculated with one‐way ANOVA, followed by Bonferroni's multiple comparison test