Figure S4.

Characterization of the scRNA-seq landscape in Ebf1^WT and Ebf1^KO LSK and LK cells. Related to Fig. 6. (A) scRNA-seq landscape derived from Ebf1^WT and Ebf1^KO LK and LSK populations, annotated for LSK and LK cells (top) and Ebf1^WT and Ebf1^KO cells (bottom). (B) UMAP visualisation of the HSC score and the expression of lineage marker genes used for the identification of cell clusters (Procr, Dntt, Elane, Klf1, Pf4, Flt3, Ly6d). (C) UMAP visualisation of the expression of Il7r and Ig genes in Ebf1^WT and Ebf1^KO cells. (D) GO enrichment analysis of the upregulated genes in cluster 4, between Ebf1^WT and Ebf1^KO cells. (E) Boxplots showing the absolute number of myeloid cells (CD11b⁺) in the BM. Ebf1^WT n = 18, Ebf1^KO n = 16, Ighm^WT and Ighm^Tm1 n = 11. Statistical significance was determined by Mann-Whitney U test. Data are from >2 independent experiments.