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. 2022 Aug 19;10:947376. doi: 10.3389/fcell.2022.947376

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Copyright © 2022 Beaven and Denholm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Achaete-scute complex genes are not required to specify distal tubule identity, but are required to expand the pool of distal identity cells. (A,B,D) ∼Stage 16 embryos stained to show the nuclei of the MpT cells (anti-Cut, brown) and nuclei from the distal MpT cells (anti-Dac, black, arrowheads). (A) Control (w ¹¹¹⁸) embryo. (B) Homozygous Df(1)sc-B57 embryo. (C) Homozygous stg ⁴ embryo. (D) Wild-type (OrR) embryo stained to show dividing cell nuclei (anti-pHH3) in relation to nuclei of the distal MpT cells (anti-Dac). For tissue context all nuclei are stained with DAPI. Single channel images equate to region in blue box of merged image. Scale bars = 50 μm.