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. 2022 Sep 1;26(5):347–355. doi: 10.4196/kjpp.2022.26.5.347

Fig. 2. FGF21 silencing inhibits HASMC proliferation, migration and phenotype switch.

Fig. 2

HASMCs were transfected with si-FGF21 or si-NC for 48 h before treatment of 100 nM Ang-II for 24 h. (A–C) RT-qPCR and western blotting for evaluating the efficiency of FGF21 knockdown in HASMCs. (D) CCK-8 assay for assessing cell proliferation in each group. (E–G) Wound healing and Transwell assays for examining cell migratory ability. Magnification for wound healing: ×40; magnification for Transwell: ×200. (H) Western blotting of SM22α and α-SMA protein expression in HASMCs. FGF21, fibroblast growth factor 21; HASMCs, human aortic vascular smooth muscle cells; NC, negative control; Ang-II, angiotensin II; RT-qPCR, real time quantitative polymerase chain reaction; CCK-8, cell counting kit-8. **p < 0.01.