FIG. 1.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunodetection of the purified FGK2 complex. The FGK2 complex was purified as described in Materials and Methods. The purified complex was then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane for immunoblotting with specific antibodies (Ab). (A) Coomassie staining of the gel. Lanes: 1, proteins eluted by glutathione; 2, proteins eluted by thrombin cleavage. (B) Immunoblotting of the purified complex with specific antibodies to GST-MalG, MalF, and MalK individually. Lanes: 1, proteins bound to the glutathione agarose resin; 2, proteins eluted by thrombin cleavage; 3, proteins retained on the column following thrombin cleavage. The values on the left are molecular weights (M.W.) in thousands.