FIG. 6.

The ATPase activity of the reconstituted FGK2 complex is MBP dependent. The purified FGK2 complex was reconstituted into proteoliposomes (as described in Materials and Methods) in the absence and in the presence of wild-type MBP. The reconstituted complex (final protein concentration, 3 μg/ml) was incubated in 40 mM Tris-HCl (pH 7)–4 mM MgCl₂–5% glycerol–4 mM ATP. For each reaction, samples were removed at various times and the amount of inorganic phosphate was determined as described in Materials and Methods. Symbols: ●, reconstituted FGK2 purified complex; ○, reconstituted FGK2 purified complex with wild-type MBP.