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Purified His6-MalK is not stimulated by MBP. The purified FGK2 complex and the purified His6-MalK protein were incubated in the absence and in the presence of different concentrations of maltose-saturated MBP. The ATP hydrolysis rates of the mixtures were determined as described in legend to Fig. 2. Symbols: ●, detergent-soluble FGK2 complex; ○, His6-MalK.
