TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| HS3309 | F−araD139 Δ(argF-lac)U169 relA1 rbsR flbB5301 ptsF25 thi-1 deoC1 malTp1Tp7 ΔmalE444zjb::Tn10-729 | Laboratory collection |
| NT169 | F−araD139 Δ(araABCD leu)7697 ΔlacX74 galU galK rpsL ΔmalB101 lacIq1rpoB | Laboratory collection |
| X90 (DE3) | F−ara Δ(lac pro) XIII gyrA argE(Am) thi rpoB | 38 |
| Plasmids | ||
| pMR31 | pACYC184 Ptac-malF+ | This work |
| pMR41 | pBR322 Ptac-gst-malG+-Ptac-malK+ | This work |
| pLH1 | pBR322 PmalB-malE+ | 17 |
| pLH38 | pBR322 PmalB-malE623 | 17 |
| pCP97 | pET15b PT7-his6-malK+ | 30 |