TABLE 2.

Effect of verapamil on the affinity of the complex for ATP and the hydrolysis rate^a

Expt no.	Km (μM)			Vmax (nmol/min/mg)
	Without verapamil	With 1 mM verapamil	% Increase	Without verapamil	With 1 mM verapamil	% Increase
1	200–400	350–500	42	550–1,000	850–1,250	36
2	160–250	250–400	58	500–720	850–1,000	52
3	125–150	200	46	550–625	850	45
4	133–200	250–350	80	1,000	1,600–2,000	80
5	133–200	200–400	80	500–600	1,000	82

^aAssays were performed in duplicate with the purified detergent-soluble FGK2 complex as described in Materials and Methods.