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. 2022 Aug 19;13:959978. doi: 10.3389/fphar.2022.959978

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Copyright © 2022 Zhang, Qin, Lyu, Lu, Chen, Fu and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Soluble and insoluble protein concentration determination of control and lanosterol treated LBs. (A) Concentration of soluble and insoluble proteins was detemined by SDS-PAGE gel electrophoresis combination with coomassie blue staining. (B) Quantitative analysis of bands around 20kd region (red box) showed that the increase of insoluble protein was less in the lanosterol treated LBs than the control LBs. Bars represent mean ± SD (n = 4). *p < .05, ***p < .001. T, total protein; S, soluble protein; I, insoluble protein. DMSO, dimethyl sulfoxide. Lano, lanosterol.