Figure 4.

Methylation of positive and reverse strands in the genome predicted using Tombo and its corresponding depth. (A) The score of 5mC and (B) 6mA modification of positive and reverse strands. We compared the raw signal levels between sequenced samples and alternative models to obtain the statistical results of the corresponding sites, Tombo generated the correlation scores to evaluate the possibility of methylation on certain sites in the full-length viral genome. A higher score indicated that the site was more likely to undergo methylation modification. (C) Depth of Nanopore sequencing of positive and reverse strands. The top 10 methylation sites and their adjacent regions in the ASFV_GZ201801_2 that are most likely to be modified by (D) 5mC and (E) 6mA methylation also predicted using Tombo.